Nanobodies and recombinant binders in cell biology
نویسندگان
چکیده
منابع مشابه
Nanobodies and recombinant binders in cell biology
Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, includ...
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purpose: the antigen binding fragments of camelids heavy chain antibodies are comprised in one single domain (vhh). the crystal structure of an isolated vhh indicated that it is a problate particle of 2.5 nm in diameter and ∼4 nm high, and has been referred to as a nanobody. the very close similarity of these molecules to human vhs illustrates the potential application of these novel products ...
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Nanobodies are recombinant, antigen-specific, single-domain, variable fragments of camelid heavy chain-only antibodies. The innate supremacy of nanobodies as a renewable source of affinity reagents, together with their high production yield in a broad variety of expression systems, minimal size, great stability, reversible refolding and outstanding solubility in aqueous solutions, and ability t...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2015
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.201409074